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hindiii digested lambda phage dna  (New England Biolabs)


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    Structured Review

    New England Biolabs hindiii digested lambda phage dna
    Hindiii Digested Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%BB+dna+hindiii+digest/HindIII/pmc13179615-117-16-20
    Average 99 stars, based on 10752 article reviews
    hindiii digested lambda phage dna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Evaluating the early diversification of Yersinia pestis and its phylogeographic expansion over 96 years of evolution in Madagascar.
    Article Snippet: Quality assessment of the resulting librarieswas donewith 2100Bioanalyzer (Agilent) before sequencing with Illumina NextSeq 500. .. ForNAU, the genomicDNA(gDNA)extractswere assessed forquality and quantity on a 0.7% agarose gel using λ DNA-HindIII Digest (New England Biolabs, Ipswich, MA, USA). .. We then sheared the gDNA to ~250 bp using a QSonica Q800 Sonicator (QSonica, Newtown, CT) at 60% amplitude, with 15 s on/off settings.

    Article Title: Evaluating the early diversification of Yersinia pestis and its phylogeographic expansion over 96 years of evolution in Madagascar
    Article Snippet: Quality assessment of the resulting libraries was done with 2100 Bioanalyzer (Agilent) before sequencing with Illumina NextSeq 500. .. For NAU, the genomic DNA (gDNA) extracts were assessed for quality and quantity on a 0.7% agarose gel using λ DNA-HindIII Digest (New England Biolabs, Ipswich, MA, USA). .. We then sheared the gDNA to ~250 bp using a QSonica Q800 Sonicator (QSonica, Newtown, CT) at 60% amplitude, with 15 s on/off settings.

    Molecular Weight:

    Article Title: A common mechanism for recruiting the Rrm3 and RTEL1 accessory helicases to the eukaryotic replisome
    Article Snippet: .. Molecular weight markers for native agarose gels were prepared by first dephosphorylating 17 μg λ DNA-HindIII Digest (New England Biolabs N3012S) with 10 U Antarctic Phosphatase (New England Biolabs M0289S) in total volume of 40 μL for 1 h at 37 °C. .. The phosphatase was inactivated by incubation at 80 °C for 10 min. 6.8 μg of dephosphorylated DNA was then labelled with γ-[ 32 P]-ATP using 40 units of T4 Polynucleotide Kinase (New England Biolabs M0201S) at 37 °C for 1 h, in a total reaction volume of 40 μL.

    Article Title: A common mechanism for recruiting the Rrm3 and RTEL1 accessory helicases to the eukaryotic replisome.
    Article Snippet: .. Molecular weight markers for native agarose gels were prepared by first dephosphorylating 17 μg λ DNA-HindIII Digest (New England Biolabs N3012S) with 10 U Antarctic Phosphatase (New England Biolabs M0289S) in total volume of 40 μL for 1 h at 37 °C. .. The phosphatase was inactivated by incubation at 80 °C for 10 min. 6.8 μg of dephosphorylated DNA was then labelled with γ-[32P]ATP using 40 units of T4 Polynucleotide Kinase (New England Biolabs M0201S) at 37 °C for 1 h, in a total reaction volume of 40 μL.

    Marker:

    Article Title: In Vitro and In Vivo Assessments of Newly Isolated N4-like Bacteriophage against ST45 K62 Capsular-Type Carbapenem-Resistant Klebsiella pneumoniae : vB_kpnP_KPYAP-1
    Article Snippet: .. The λ DNA-HindIII Digest (New England Biolabs) marker was used to compare the size of the digested fragments. .. The phage DNA genome was sequenced by AllBio.

    Long Range PCR:

    Article Title: A Staphylococcus capitis strain with unusual bacteriocin production
    Article Snippet: .. Slot 2, long range PCR product of S. capitis BN2; slot 4, long range PCR product of S. capitis NPG517; slot 5, λ DNA EcoRI digest (bands at 21, 7.4, 5.8, 5.6, 4.9, and 3.5 kb); slot 6, Quick-Load Purple 1 kb DNA ladder (NEB #NO552G0); slot 7, λ DNA HindIII digest; slot 8-13 long range PCR of non-producer clones; slot 14, 1 μl Quick-Load Purple 1 kb DNA ladder (NEB). ..

    Clone Assay:

    Article Title: A Staphylococcus capitis strain with unusual bacteriocin production
    Article Snippet: .. Slot 2, long range PCR product of S. capitis BN2; slot 4, long range PCR product of S. capitis NPG517; slot 5, λ DNA EcoRI digest (bands at 21, 7.4, 5.8, 5.6, 4.9, and 3.5 kb); slot 6, Quick-Load Purple 1 kb DNA ladder (NEB #NO552G0); slot 7, λ DNA HindIII digest; slot 8-13 long range PCR of non-producer clones; slot 14, 1 μl Quick-Load Purple 1 kb DNA ladder (NEB). ..

    Molecular Cloning:

    Article Title: Universal nucleic acid preservation in biological fluids with boron clusters
    Article Snippet: Barnase (cat no. MBS1146186) and Barstar (cat no. MBS1053017) were purchased from MyBioSource. .. RNase inhibitor was purchased from Molecular Cloning Laboratories (cat no. RNIN200). λ DNA-HindIII Digest (cat no. N3012) and dsRNA Ladder (cat no. N0363S) were purchased from New England BioLabs. λ DNA was purchased from Takara Bio (cat no. 3010). ssRNA was purchased from Millipore (cat no. 55714). tRNA (cat no. 10109495001) and MS2 single-stranded RNA genome (cat no. 10165948001) were purchased from Roche. .. Proteinase K was purchased from New England Biolabs (cat no. P8107S).



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